Nonclinical Services


Safety Pharmacology Studies

In LSIM Safety Institute, safety pharmacology studies on pharmaceuticals, medical equipment, regenerative medicine products, agricultural chemicals, veterinary drugs, etc. can be conducted under various GLP and/or guidelines. Most of the animal laboratory personnel who are qualified as laboratory animals 1st, 2nd grade technicians or JACL certified technicians, have highly reliable technique based on abundant experience, and have quick and detailed analysis ability.

Our Service

  • 1.Safety pharmacology studies of pharmaceuticals

    • 1.1Safety pharmacology core battery
      • Central nervous system

        • 1Effect on general activity and behavior (Modified Irwin's method/FOB)

        • 2Effect on spontaneous locomotive activity

        • 3Effect on body temperature

      • Cardiovascular System

        • 1Effect on the blood pressure, heart rate and ECG in conscious and unrestrained dogs or monkeys (Telemetry method)

        • 2Effect on Ikr current (Whole cell/patch clamp method; hERG transduced cells)

        • 3Effects on myocardial action potential (Microelectrode technique; isolated papillary muscle)

      • Respiratory System

        • 1Effect on the respiratory rate and tidal volume in conscious and unrestrained animals (Whole Body Plethysmography)

        • 2Effect on the respiratory rate and arterial blood gases in conscious and unrestrained animals

          * To comply with safety pharmacology studies on regenerative medicine products, effects on the central nervous system (Modified Irwin's method), blood pressure and heart rate (Tail cuff method) and respiratory function (Whole body plethysmography) can be evaluated by using NOG mice and NOD SCID mice.

    • 1.2Follow-up study
      • Central Nervous System

        • 1Learning and memory (passiveness avoidance response, Morris water maze.)

        • 2Effects on muscle coordination (Rotarod method)

        • 3Effect on anesthesia (Thiopental-induced sleep, pentobarbital-induced sleep)

        • 4Synergic and antagonistic effects of convulsions (Electric shock-induced seizures, Pentetrazol-induced convulsions)

        • 5Effects on algesthesia (Tail flick, tail pinch, Randall-Selitto test, Acetic acid-induced writhing)

        • 6Muscular relaxation (Suspension, inclined plate method)

      • Cardiovascular System

        • 1Effects on the blood pressure, heart rate and ECG in anesthetized animals

        • 2Measurement of left ventricular internal pressure in conscious dogs or monkeys (Telemetry method)

        • 3Evaluation on chemical-inducing orthostatic hypotension using telemetry method

    • 1.3Supplemental Safety Pharmacology Studies
      • Renal/Urinary System

        • 1Effects on urine volume and urinary electrolyte measurement experiment

      • Autonomic Nervous System

        • 1Effect on intestinal transport ability

        • 2Effect on automatic motility of isolated ileum

        • 3Effects on secretion of gastric juice and saliva

        • 4Effects on gastric and duodenal mucosa

        • 5Effect on gastrointestinal motility (telemetry method)

        • 6Effect on gastric pH

        • 7Effect on gastric emptying capacity

      • Blood System

        • 1Hemolysis

        • 2Platelet aggregating action (ADP induction, collagen induction)

        • 3Blood coagulation (PT, APTT, bleeding time)

      • Somatic Nervous System

        • 1Skeletal muscle (sciatic nerve-gastrocnemius tendon reflex, excised diaphragm test etc.)

        • 2Local anesthesia (corneal reflex, skin reflex)

      • Other Organ Systems

        • 1Effects on blood vessels, trachea, vas deferens, uterus, ileum, diaphragm (isolated organs)

  • 2.Elucidation of the action mechanism of agrichemicals

    • 2.1Symptom observation

    • 2.2Central nervous system

    • 2.3Respiratory and circulatory system

    • 2.4Renal function

    • 2.5Autonomic nervous system

    • 2.6Skeletal muscle

    • 2.7Blood system

    • 2.8Digestive system

Contact Us

We provide information for clients concerning development of pharmaceuticals, agrichemicals, and chemical substances etc.
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